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Contact
Info: |
Courier
Address: |
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Phone:
(203) 785-4055
Fax:
(203) 785-4951
e-mail:
emile.boulpaep@yale.edu
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333
Cedar Street
SHM
B142
New
Haven,
CT 06510 |
Membrane
channels and transporters in kidney.
Polarized
kidney tubule cells have proteins in the plasma membrane facing the
urine that differ from those facing the blood. We are studying the electrical
characteristics of these proteins-ion channels and electrogenic transporters-in
the two plasma membranes of renal tubule cells. Patch-clamp, optical
and molecular biology techniques are being used.
1.)
We study the physiological role and regulation of single small-conductance
chloride channels, which are activated by cAMP through phosphorylation
by protein kinase A. Our interest is in the intracellular signal transduction
pathways involved in the regulation of these renal channels and their
homology with the CFTR protein, known to be deficient in cystic fibrosis.
2.)
We study the physiological role and regulation of a cGMP-sensitive non-selective
cation channel in the apical membrane of a kidney cell line and its
homology with a cGMP-gated non-selective channel of the vertebrate photoreceptor.
3.)
Using confocal vital microscopy and ion-sensitive fluorescent dyes,
we study a model of proximal tubule cell injury induced by angiotensin
II, which is accompanied by elevation of intracellular calcium and plasma
membrane blebbing.
Figure caption:
Confocal
microscopy phase contrast image of a single proximal tubule cell isolated
from Ambystoma kidney. The cell maintains its epithelial polarity, with
a prominent brush-border visible along the apical membrane. Isolated
cells are used for patch-clamp studies of ion channels and for monitoring
intracellular free Ca++ concentration by means of fluorescent
indicators.
Selected
publications:
Click
 for
PDF
Choi
I, Aalkjaer C, Boulpaep EL, Boron WF. An electroneutral sodium/bicarbonate
cotransporter NBCn1 and associated sodium channel. Nature. 2000 Jun
1;405(6786):571-5. 
Haitham T. Idriss, Yusuf A. Hannun, Emile Boulpaep and Srisaila Basavappa.
Regulation of volume-activated chloride channels by P-glycoprotein:
phosphorylation has the final say? J.Physiol. 2000, 524:629-636. 
Schmitt BM, Biemesderfer D, Romero MF, Boulpaep EL, Boron WF. Immunolocalization
of the electrogenic Na+-HCO-3 cotransporter in mammalian and amphibian
kidney. Am J Physiol. 1999 Jan;276(1 Pt 2):F27-38. 
emile.boulpaep@yale.edu
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