The Richard D. Frisbee III Laboratory of Stem Cell Transplantation and Hematopoietic Graft Engineering, located in the Transfusion Medicine Section of the Department of Laboratory Medicine, is a state-of-the-art class 10,000 clean room facility. In a class 10,000 laboratory, there are fewer than 10,000 particles per cubic foot of air. This laboratory was designed with the goal of performing clinical scale human stem cell isolation and manipulation that will improve upon existing treatments for cancer and hematological diseases. The room was completed in September, 1998, and is used for human gene therapy protocols, hematopoietic stem cell isolation via immunomagnetic CD34+ cell selection, and T cell depletion for mismatched allogeneic transplantation. Other uses include ex vivo expansion of human hematopoietic stem cells. The Laboratory was funded predominantly through a grant from the Richard D. Frisbee III Foundation.